DNA

Part:BBa_K741000:Design

Designed by: Wuyang Chen   Group: iGEM12_USTC-China   (2012-07-23)

anticro


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 134
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We construct this part by PCR with design primers which can reversely arrange the sites for EcoRI, XbaI, SpeI, PstI. The template DNA we use is the genome of lambda phage.

You need a promoter and a terminator but no RBS when using this part.

Source

Genome of lambda phage

References